Waste from so-called ‘mirror entries’ in the list of wastes must be categorised as ‘hazardous’ or ‘non-hazardous’ depending on its composition. Hazardous properties are determined on the basis of the concentrations of the waste constituents or on the basis of a test. For the hazard characteristic ‘HP 14’ (ecotoxic), there are currently no specific requirements at EU level for categorisation based on tests (bioassays). For this reason, a recommendation on the ecotoxicological characterisation of waste was published by ⁠UBA⁠ in 2013. The aim of the project was to draw up proposals for updating and further developing this recommendation on the basis of ecotests. Furthermore different test strategies were compared.