Waste from so-called ‘mirror entries’ in the list of wastes must be categorised as ‘hazardous’ or ‘non-hazardous’ depending on its composition. Hazardous properties are determined on the basis of the concentrations of the waste constituents or on the basis of a test. For the hazard characteristic ‘HP 14’ (ecotoxic), there are currently no specific requirements at EU level for categorisation based on tests (bioassays). For this reason, a recommendation on the ecotoxicological characterisation of waste was published by UBA in 2013. The aim of the project was to draw up proposals for updating and further developing this recommendation on the basis of ecotests. Furthermore different test strategies were compared.