The UBA aims to contribute to the development of an EU guidance document on risk assessment for amphibians. As part of the project, data on the toxicity of various pesticides to amphibians were systematically researched, processed, and supplemented with its own experiments to establish a scientific basis for risk assessment. The impact of direct exposure through overspraying of amphibians and indirect exposure via sprayed soil substrate were investigated. Furthermore, correlations between mortality, effects on skin and organs, and physicochemical parameters of the active ingredients, as well as their toxicity to other species (birds, mammals, aquatic organisms, earthworms), were examined.